Villegas Silva, V.A., M., Muñoz Ochoa, Y.E., Rodríguez Montesinos & D.L., Arvizu Higuera (2022). Partial characterization and biological activity of Sargassum lapazeanum (Fucales, Phaeophyceae). Hidrobiológica. 32(3): 365-372.
Valeria Alexandra Villegas Silva 1, Mauricio Muñoz Ochoa 2, Yoloxochilt Elizabeth Rodríguez Montesinos 2 y Dora Luz Arvizu Higuera 2
Background.The Baja California peninsula contains a wide variety of habitats and climatic conditionsand is thus home to an enormous wealth of marine algae. Algae belonging to the Sargassum genus are often dominant in communities of the Gulf of California dueto their high biomass. Goals. This study aimed to evaluate the potential of Sargassum lapazeanum as a source of compounds with antioxidant, antibacterial, or anticoagulantactivity in addition to its proximal composition and alginate content. Methods.An ethanolic extract (EE) was obtained from dried algae to evaluate antibacterialactivity by means of the disc agar diffusion method and antioxidant activity bythe stable free radical method (DPPH). The crude fucoidan (CF) obtained was fractionatedby different chromatographic techniques, and its anticoagulant activity wasevaluated by prothrombin time (PT) and activated partial thromboplastin time(APTT) assays. A structural characterization was also conducted. In addition, theproximal composition and alginate content and quality were determined. Results.The EE and its different fractions showed selective activity against bacteriaof the Vibrio genus, such as V. harveyi and V. parahaemolyticus, inaddition to free radical scavenging activity. The lowest half maximal effectiveconcentration (EC50 = 39.96 µg mL-1) was obtained from fraction F6.Crude fucoidan and its fractions showed anticoagulant activity, withvalues comparable to those observed forheparin at concentrations of 10 µg mL-1. The sodium alginate yieldwas 22.8%, with low viscosity and high gel strength. Conclusions. Sargassumlapazeanum has high potential to be a source of compounds with antioxidant,antibacterial, and anticoagulant activity.
Palabras clave: biological activity; brown seaweed; phytochemistry; polysaccharides; Gulf of California.
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