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Resumen del producto

Hernández-Sandoval, F.E., J.J., Bustillos-Guzmán, C.J., Band-Schmidt, E.J., Nuñez-Vázquez, D.J., López-Cortés, L.J., Fernández-Herrera, C.A., Poot-Delgado & M., Moreno-Legorreta (2022). Effect of different N:P ratios on the growth, toxicity, and toxin profile of Gymnodinium catenatum (Dinophyceae) strains from the Gulf of California. Toxins. 14(7): 501. DOI: 10.3390/toxins14070501.

Effect of different N:P ratios on the growth, toxicity, and toxin profile of Gymnodinium catenatum (Dinophyceae) strains from the Gulf of California

Francisco Eduardo Hernández-Sandoval, José Jesús Bustillos-Guzmán, Christine Johanna Band-Schmidt 1, Erick J. Nuñez-Vázquez, David Javier López-Cortés, Leyberth José Fernández-Herrera, Carlos A. Poot-Delgado y Manuel Moreno-Legorreta

1 Instituto Politécnico Nacional, Centro Interdisciplinario de Ciencias Marinas
The harmful microalgae Gymnodinium catenatum is a unique naked dinoflagellate thatproduces paralytic shellfish poisoning toxins (PSTs). This species is common along the coasts of theMexican Pacific and is responsible for paralytic shellfish poisoning, which has resulted in notablefinancial losses in both fisheries and aquaculture. In the Gulf of California, G. catenatum has beenrelated to mass mortality events in fish, shrimp, seabirds, and marine mammals. In this study, thegrowth, toxin profiles, and toxin content of four G. catenatum strains isolated from Bahía de LaPaz (BAPAZ) and Bahía de Mazatlán (BAMAZ) were evaluated with different N:P ratios, keepingthe phosphorus concentration constant. All strains were cultivated in semi-continuous cultures(200 mL, 21.0 C, 120 mol photon m??2s??1, and a 12:12 h light-dark cycle) with f/2 + Se mediumusing N:P ratios of: 4:1, 8:1, 16:1, 32:1, and 64:1. Paralytic toxins were analyzed by HPLC withfluorescence detection. Maximum cellular abundance and growth were obtained at an N:P ratioof 64:1 (3188 cells mL??1 and 0.34 div day??1) with the BAMAZ and BAPAZ strains. A total of tensaxitoxin analogs dominated by N-sulfocarbamoyl (60–90 mol%), decarbamoyl (10–20 mol%), andcarbamoyl (5–10 mol%) toxins were detected. The different N:P ratios did not cause significantchanges in the PST content or toxin profiles of the strains from both bays, although they did affectcell abundance.

Palabras clave: Gulf of California; Gymnodinium catenatum; N:P ratio; paralytic toxins; semi-continuous culture; toxin profile

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