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The lack of a suitable food for octopuses in the early stages post hatching (PH) is a limitation for their commercial aquaculture. Knowing the use of nutritional reserves and the activity of digestive enzymes during this stage allows developing and implementing specific diets. The Pacific Pygmy Octopus Paroctopus digueti was used as a model due to its direct development and quick growth, with high potential for culture. Brooding females were collected from the natural environment, which were placed in conditions of captivity until the hatching of the eggs. The obtained hatchlings were kept in individual containers, fed with fresh crab meat and samples were taken the day of hatching and every two days until day 14. The digestive gland (DG) was isolated, as well as muscle (M) from the arms an evaluation of alkaline and acidic proteolytic activity, lipases in DG. The quantification of soluble protein and glycogen content in DG and M, were done. The results obtained from the activity of digestive enzymes (acidic proteases !¯ = 4656 ± 825.8 U mg protein-1 day 4 PH) and the content of nutritional reserves of P. digueti help us to observe an apparent process of adaptation to exogenous food and a mobilization of reserves between day 2 and 4 after the hatching (soluble protein GD !¯ = 27.9 ± 0.9 mg-1 day 4 PH), which may reflect a high metabolism and dependence of exogenous food as has been observed in other species of octopuses with direct development. These results bring us closer to find a formulated diet for this octopus, which would help to diversify the culture octopus species.

Palabras clave: Lipases; Proteases; Glycogen; Soluble protein; Octopus culture

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