Resumen del producto

Pelagic copepods are maxilopods crustaceans that due to its abundance and frequency are an important link in the marine food web. Their great diversity demand accuracy in the species identification, because in some cases they share morphometric and meristic features which tend to confuse the identity of the species. To identify specimens key species usually are used; however, since late 20th century molecular tools for the study of biodiversity has been used, specifically through the use of a fragment of DNA with cytochrome c oxidase I (COI) gene. As part of a broader project, presents the advance of the identification of copepods through DNA barcoding. Copepods were obtained with a bongo net with 500 um filter mesh, preserved with ethyl alcohol at 100%; individuals were picked out from samples and identified to species, and processed to DNA extraction and sequencing in the Chetumal Node of the National Laboratory of Barcode and Canadian Centre for DNA Barcoding (University of Guelph), respectively. Sequences were lined up with MEGA 5 and GenDoc software, in order to get the tree of similarity using Neighbor Joining (NJ) algorithm. 27 Copepod species which has genetic sequencing and subsequently a tree similarity was built, and we observed that all species were separated with at least 2% of divergence. It is currently in the process more than 120 specimens belonging to at least 30 species obtained in different geographic locations within the Gulf of California and Marias islands.

Palabras clave: Biorepositories and Collections; Data Analysis; Marine Barcoding; Barcoding Biotas

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