Resumen del producto

The research of bacteria associated to marine sponge, is interesting due to the possibility for discovery of new source for bioactive compounds. Actinomycetes are an important group being a rich source of compounds with a high pharmacological potential. Therefore we focused our investigation in the isolation and cultivation of sponge bacteria with antibacterial potential. In this study of sponge-associated bacteria, 12 strains of actinomycetes were isolated. The sponges were collected in Punta Arena de la Ventana (A. gerardogreeni) and Pichilingue (Haliclona sp, Mycale sp.), B.C.S. by SCUBA diving. Isolation and purification of the strains was performed on TSA agar plates with 2.7% NaCl, which were incubate for 3 to 5 days at room temperature. Therefore, grown colonies were selected and each of them was plated again for purity check and morphological and microscopical description. Resulting colonies were grown for the biomass production for DNA extraction. The strain identification was performed using 16 RNAr gene amplification by PCR and subsequent sequencing. The sequences were compared with the GenBank database. Micrococcus luteus was isolated from A. gerardogreeni sponge, Nocardioides sp. and Corynebacterium sp. from Haliclona sp. sponge, while Micrococcus sp., Kocuria sp. and Brachybacterium sp. were isolated from Mycale sp. A preliminary antibacterial assay showed that only strains isolated from Mycale sp. showed inhibitory activity against pathogenic resistant bacteria Staphylococcus aureus and Escherichia coli.

Palabras clave: sponges; Body growth; actinomycetes; isolation

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