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Resumen del producto
Gorrostieta-Hurtado, E.I., E., Michel-Morfín, M.B., Aguilar-Ramirez, A., Falcón-Alcantara, L., Palma-Tirado, F.A., García Domínguez & E.P., Heimer de la Cotera
(2011).
Anatomical, histological and chromatographic characterization of the venomous apparatus of Conus purpurascens.
44th Western Society of Malacologists Annual Meeting and 12th National Biennial Meeting of Malacology and Conchology of The Sociedad Mexicana de Malacología, A.C..
La Paz, Baja California Sur, México, México, junio 27 - 30, 2011,
142.
Anatomical, histological and chromatographic characterization of the venomous apparatus of Conus purpurascens
Erendira I. Gorrostieta-Hurtado, E. Michel-Morfín, M.B. Aguilar-Ramirez, A. Falcón-Alcantara, L. Palma-Tirado, Federico Andrés García Domínguez y E.P. Heimer de la Cotera
Conus purpurascens is a fish hunting snail; the conotoxins in their venom inhibit the muscles of vertebrates and have paralytic effects. The poison is found primarily in the venom duct, but it has been reported that the tooth can be preloaded with venom from the radular sac and the bulb may be involved in the biosynthesis of the poison (Safavi-Hemami, 2010). The present work aims to examine the external anatomy, histology, and chromatographic composition of some structures of the venom apparatus. Specimens of C. purpurascens were collected in Barra de Navidad, Jalisco, Mexico and the venomous apparatus was obtained to describe its anatomy. Radular sac, esophageal ring and bulb were processed through critical drying point for transmission electron microscopy (TEMP) observation; venom duct, esophageal ring and bulb were processed histologically (15 microns thick and stained with toluidine blue). Finally, we obtained the crude extract of the bulb, venom duct and radular sac and they were subjected to high-performance liquid chromatography (HPLC) to separate proteins constituting each of the tissues. The bulb contained muscle, connective tissue and protein granules in the lumen, which were also observed by electron microscopy. The center of the bulb was crossed by a duct which began at the distal end and terminating in the venom duct. The poison duct consisted of connective and epithelium tissue with unicellular glands. The radular sac and radular tooth contained venom. Venom duct and radular sac were attached to the esophageal ring consisting of muscle and connective tissue. The chromatographic profile showed that the contens of the venom duct, radular sac and bulb were peptides or proteins, that may show activity, as has been shown in other studies with the substance obtained by milking of this species (López-Vera, 2007; Möller y Mary, 2010). The data support the hypothesis that the radular teeth are preloaded on the radular sac and the biosynthesis of venoms in the bulb (Marshall, 2002; Safavi-Hemami, 2010).
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