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Rosales Velázquez, M.O., R.I., Ochoa Báez, W.M., Contreras S., J.L., Ortiz Galindo, T., Grayeb del Alamo & V., Carrasco Chávez (2009). Effects of 17?-methyltestosterone on gonadal sex differentiation in spotted sand bass (Paralabrax maculatofasciatus). Aquaculture America 2009. Seatle, Washinton, USA, Estados Unidos de América, febrero 15 - 18, 2009, 294.

Effects of 17?-methyltestosterone on gonadal sex differentiation in spotted sand bass (Paralabrax maculatofasciatus)

Martín Oscar Rosales Velázquez, Rosa Isabel Ochoa Báez, Wilfrido M. Contreras S., José Luis Ortiz Galindo, Tanos Grayeb del Alamo y Victor Carrasco Chávez

Knowledge of a species for aquacultural purposes include diverse factors that are must be studied and understood to detail. One of these factors is the way that sexual determination takes place in a species and the plasticity of manipulating sex differentiation in order of obtaining single sex populations. Production of monosex populations has two objectives: to obtain the sex with faster growth and avoid reproduction to direct energetic budget to somatic growth. Due to its protoginic condition, for the spotted sand bass (Paralabrax maculatofasciatus) we have proposed the use of all male populations. In the present study oral administration of 17 ?-Methyltestosterone (MT; Sigma No. M-7252) was used. This is the most common method to achieve masculinization, favoring testis development. The most relevant result was the gonadosomatic index (GSI), in which significant difference of the fish control with all was the originating fish of the treatments (Figure 1). This reflects the effect of the MT in the gonad development, where presumably the used doses were excessive, having like consequence gonads with low developed, underdeveloped or not formed (Figure 2). In conclusion, of all the treatments and times used, the one of 15 mg/Kg (15 MT) and 30 days of exhibition was sufficient to obtain the raised objectives, obtaining in addition with this dose, a 50% of sterility, which the aims that were looking in order to avoid the reproduction were adequate.

Palabras clave: a-amylase; sex diferentiation; esteroids; Masculinization

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