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Smith, L.M., S.M., Williams, B., Lopes da Silva Ferrette, B.J., Holmes, N., Isigi Kadagi, C.P., Lu, S., Ortega-García, J.G., Pepperell, I.R., Tibbetts, N., Wambiji, S., Wambua & C.L., Dudgeon (2025). Global analyses of sailfish (Istiophorus platypterus) using next-generation sequencing reveal multiple populations. ICES Journal of Marine Science. 82(12): fsaf232. URL.

Global analyses of sailfish (Istiophorus platypterus) using next-generation sequencing reveal multiple populations

Laura M. Smith 1, Samuel M. Williams 2, Bruno Lopes da Silva Ferrette 3, Bonnie J. Holmes 4, Nelly Isigi Kadagi 5, Ching-Ping Lu 6, Sofía Ortega-García 7, Julian G. Pepperell 8, Ian R. Tibbetts 1, Nina Wambiji 9, Sammy Wambua 10 y Christine L. Dudgeon 11

1 The University of Queensland,School of the Environment
2 Queensland Department of Primary Industries
3 Universidade Estadual Paulista Julio de Mesquita Filho
4 University of the Sunshine Coast
5 World Wildlife Fund
6 National Taiwan Ocean University
7 Instituto Politécnico Nacional, Centro Interdisciplinario de Ciencias Marinas, Pesquerías y Biología Marina
8 Pepperell Research and Consulting
9 Kenya Marine and Fisheries Research Institute
10 Pwani University
11 The University of Queensland, School of Biomedical Sciences

The sailfish ( Istiophorus platypterus ) is a mobile epipelagic billfish whose range extends across the world’s tropical and subtropical oceans. Once thought to be two allopatric species, respectively inhabiting the Indo-Pacific and Atlantic oceans, molecular analyses support a single species with global distribution. Adequate sampling of widespread pelagic species presents considerable challenges, and most previous studies on sailfish used small numbers of molecular mark er s. As such, our under standing of their global population structure was limited. In this study, we collaborated with fisheries researcher s and fisher s to build a comprehensive genomic dataset of single-nucleotide polymorphisms for sailfish spanning most of its range. Here, we examined genetic variation using three filtering approaches: (i) the full-loci dataset, (ii) putatively neutral loci, and (iii) large- FST loci for 590 sailfish from 20 locations to explore contem- porary population structure and connectivity in a global context. Cluster analyses of all datasets indicated three discrete populations: the Atlantic, eastern Pacific, and Indo-West Pacific oceans. For the first time, sailfish sampled from locations across the Indo-West Pa- cific revealed genetic connectivity throughout this region. Analyses of a subset of large- FST loci suggested a small reduction in gene flow between the western and eastern Atlantic Ocean and between the western Indian Ocean and the rest of the Indo-West Pacific. These insights into contemporary population structure can inform future stock assessments and cross-jurisdictional management of this migratory marine species.

Palabras clave: Genomic analysis; Sailfish; global populations

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