Peña, R., S., Bernal-Cerino, L., Burciaga-Cifuentes & E., Zúñiga-Villarreal (2024). Effects of probiotics on growth and biological performanceof long snout seahorse Hippocampus reidi. International Symposium on Fish Nutrition and Feeding. Puerto Vallarta, México, mayo 27 - 31, 2024, 94-94.
Renato Peña 1, Samuel Bernal-Cerino 2, Luis Burciaga-Cifuentes 3 y Eliezer Zúñiga-Villarreal 4
The long-snouted seahorse Hippocampus reidi is one of the most traded seahorse species worldwide. Culture under controlled conditions is an alternative to supply the demand for the ornate industry and traditional medicine. H. reidi has been cultured for commercial purposes; however, the effect of probiotics during the early culture has not been evaluated. Therefore, the objective of the present study was to evaluate the effect of probiotics on the growth, survival, and feeding efficiency of juvenile H. reidi using a commercial product (Sanolife MICTM; INVE, Thailand) which consists of a mixture of Bacillus subtilis, Bacillus licheniformis, and Bacillus pumilus (5x1010 cfu g-1) previously used in marine fish larviculture. Thirty juveniles H. reidi (2 months old) were donated by the commercial farm INGENS Cultivos Marinos in Mazatla´n, Mexico. They were distributed into six 20 L rectangular plastic tanks (5 juveniles per tank) and two treatments were tested in triplicate for 60 days. In one treatment, the probiotics (P treatment) were added to the tanks once daily in the morning before feeding, at a concentration of 1x109 cfu (20 mg tank- 1); and in the other tanks, no probiotics were added as a control (C treatment). The juveniles were fed with adult Artemia ad libitum twice a day (9:00 and 16:00 hrs). Our results showed that the addition of probiotics had a significant impact on the growth, survival, activity level, and feeding efficiency of the juvenile H. reidi. At the end of the study, the juveniles in the P treatment were significantly larger (LS = 10.37 ? 0.71 mm) and heavier (W = 2.98 ? 0.71 gr) than the juveniles in the control group (LS = 9.46 ? 1.18 mm and W = 1.96 ? 0.68 mm). Also, the juveniles of the P group had a higher condition factor (k) (0.26) than the juveniles of the control group (0.22). The seahorses of the P treatment spend more time swimming and displayed a higher number of attacks (23.50 attacks/5min) and consumed a higher number of preys (19.25/5 min) with a feeding success of 0.81. On the other hand, the juveniles in the C group spent more time in the bottom of the tank without swimming and only displayed 4.25 attacks/5 min and consumed an average of 4.25 preys for a feeding efficiency of 1. Additional studies are required to elucidate the effect of the probiotics on the bacterial community of the seahorses and the seawater.
Palabras clave: long snout seahorse; probiotics; Hippocampus reidi; Growth
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