Miranda-Gastelum, L.G., A.R., Díaz-Marrero, J.J., Fernández-Castro, C.J., Band-Schmidt, J.M., Borges Souza & C.J., Hernández Guerrero (2024). Antifouling activity and chemical composition of the sponge Haliclona caerulea. 4o. Biotechnology World Symposium. Cancún, Quintana Roo, México, octubre 7 - 11, 2024, 28.
Leticia Guadalupe Miranda-Gastelum 1, Ana Raquel Díaz-Marrero 2, José Javier Fernández-Castro 3, Christine Johanna Band-Schmidt 4, José Manuel Borges Souza 5 y Claudia Judith Hernández Guerrero 1
Marine fouling, due to deterioration and cleaning costs, is a problem for the shipping industry. To avoid this problem, highly toxic paints are used. As an ecological alternative, the use of marine compounds with antifouling activity has been proposed. Previous studies have shown that the extract of the sponge Haliclona caerulea has antifouling activity against bacteria. In this work, we tested the toxicity and antifouling activity of four fractions of the sponge (AcOEt, DCM, MeOH, and BuOH). And the chemical composition of the most active fraction was analyzed. The results show that the fractions were not toxic in the Artemia franciscana model (LC50 > 1500 µg mL-1). DCM and BuOH fractions (100 µg mL-1) moderately inhibited the growth of biofilm-forming bacteria. BuOH and MeOH fractions (50 µg mL-1) were able to reduce biofilm formation in 71%. The MeOH fraction was purified by chromatography (SEC and RP-HPLC), the 1HNMR, 13C-NMR, COSY and HSQC spectra indicate the presence of a heteroaromatic amine. It is expected that the identified compounds will be able to be used as non-toxic antifouling agents in laboratory and field tests.
Palabras clave: extracts; cromatography; marine compounds
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