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Hernández Guerrero, C.J., R., Dronne, D., Pérez-Solis, L.G., Miranda-Gastelum & E.T., Quintana (2024). Marine biofilm-forming bacteria from La Paz Bay, Gulf of California. 4o. Biotechnology World Symposium. Cancún, Quintana Roo, México, octubre 7 - 11, 2024, 52.

Marine biofilm-forming bacteria from La Paz Bay, Gulf of California

Claudia Judith Hernández Guerrero 1, Rejane Dronne 2, Danisley Pérez-Solis 3, Leticia Guadalupe Miranda-Gastelum 4 y Erika T. Quintana 5

1 Instituto Politécnico Nacional, Centro Interdisciplinario de Ciencias Marinas, Desarrollo Tecnologías
2 Université de Bregtane Occidentale
3 UNIVERSIDAD POLITÉCNICA DE PUEBLA
4 Instituto Politécnico Nacional, Centro Interdisciplinario de Ciencias Marinas, Desarrollo de Tecnologías
5 Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas

The production of biofilm by bacteria is the early stage of microfouling and it will eventually support macrofouling. Biofilm allows macro-organisms to attach and settled on submerged inert substrates and this has negative direct effects in the maritime industry. It is important to isolate, to cultivate and to identify the bacteria involved in marine biofilm, so that specific antifouling strategies may be design. The aim of this research was to isolate, cultivate and identify bacteria with capacity to form a biofilm in zones of marinas and other locations from La Paz Bay in the southern Gulf of California. The bacteria were isolated from inert substrates (wood, acrylic and zinc), cultivate on marine agar plates and identified by 16S rRNA sequencing. Biofilm-forming ability was assessed by a microplate assay using crystal violet staining. Seventeen bacteria with the capacity to form biofilms under laboratory conditions were identified. The identified genera were: Alteromonas (5), Vibrio (7), Bacillus (2), Antarcticimicrobium (1), Pseudoalteromonas (1) and Ruegeria (1). This collection can be used as model microorganisms in assays of antifouling compounds that allow the design of more effective control strategies.

Palabras clave: Bacteria; isolation; microfouling

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