Pérez Pérez, J.M., R.N., Águila Ramírez, I., Leyva Valencia & C.J., Band Schmidt (2024). Antimicrobial evaluation of the methanolic extract of Amphidinium carterae. 4th Biotechnology World Symposium. Cancún, México, octubre 7 - 11, 2024, 309.
Jennyfer Marisol Pérez Pérez, Ruth Noemí Águila Ramírez 1, Ignacio Leyva Valencia 1 y Christine Johanna Band Schmidt 1
Dinoflagellate species of the genus Amphidinium are producers of lipophilic compounds called amphidinols, reported to have activity against Micrococcus luteus, Bacillus subtilis, Staphylococcus aureus, Escherichia coli and Candida albicans. This study aimed at evaluating the bioactivity of the methanolic extract of ACCETMAR-2 from Amphidinium carterae to inhibit the growth of pathogenic bacteria. This strain was obtained from the dinoflagellate collection of CICIMAR-IPN, and was scaled up to 6 L. The biomass was separated from the supernatant by centrifugation, was lyophilized, and sonicated with methanol to recover methanolic extracts by centrifugation and concentrated under reduced pressure. The antimicrobial and minimum inhibitory concentration bioassays were performed by the disk diffusion method. Ten strains of pathogenic bacteria, three gram-negative, seven gram-positive, and one yeast, were evaluated at 10 concentrations. It was observed that the extracts were active against Staphylococcus epidermidis and Aeromonas caviae with a minimum inhibitory concentration of 31.25 µg/mL, and against the yeast Candida albicans, at the concentration of 1 mg/mL. It is concluded that the methanolic extract of ACCETMAR-2 strain has activity against gram positive and negative bacteria, and against Candida albicans.
Palabras clave: dinoflagellate; Antimicrobial; Natural products
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