Rico-Virgen, E.G., R.N., Águila-Ramírez, M., Muñoz-Ochoa & F., Vargas-Betancourt (2024). Production and characterization of antimicrobial peptides induced by thermal stimulation. 4th Biotechnology World Symposium. Cancún, México, octubre 7 - 11, 2024, 92.
Erika Guadalupe Rico-Virgen, Ruth Noemí Águila-Ramírez 1, Mauricio Muñoz-Ochoa 1 y Francisco Vargas-Betancourt
One of the most stressful factors for bacteria is temperature, as it can slow down metabolism and induce the production of diverse peptides that promote their survival, either as an adaptive or temporary response. In this context, a pre-inoculum of the Bacillus firmus strain, isolated from an intertidal zone, was exposed at a temperature of 4° C for 72 hours. Subsequently, the culture was scaled up (100 mL/44 h/30° C) to obtain a cell-free supernatant, which was precipitated with (NH4)2SO4 (80%) and dialyzed in PBS using a 2 kDa cut-off membrane to obtain a crude extract. Bioactive peptides with a molecular weight less than 10 kDa were detected, these peptides are heat-resistant (more than 100° C), tolerate a wide pH range (2-11) and resist exposure to UV radiation. A partial fractionation by molecular size was performed by ultrafiltration, where the 2-9 kDa fraction showed the presence of three zones of inhibition in the bioautographic assay against aquaculture phatogens. The results confirm the capacity of B. firmus as a producer of multiple active peptides with characteristics similar to bacteriocin-like substances.
Palabras clave: Antimicrobial; peptide; Bacteria
Para obtener una copia del documento contacta la personal de la biblioteca a través del correo bibliocicimar{a}ipn.mx