Pedraza-Chávez, M.N., R.J., Delgado-Macuil, V.H., Cruz-Escalona & R.N., Águila-Ramírez (2024). Development of biosensors for the determination of heavy metals in stingray mucus. 4th Biotechnology World Symposium. Cancún, México, octubre 7 - 11, 2024, 72.
Mayra Nelly Pedraza-Chávez 1, Raúl Jacobo Delgado-Macuil 1, Víctor Hugo Cruz-Escalona 2 y Ruth Noemí Águila-Ramírez 2
Heavy metals are chemical elements with high atomic weight and density, they are important pollutants because they are potentially toxic and are not degradable and they tend to bioaccumulate in the environment where they are found. The objective of this work was to develop optical biosensors from silicon wafers, using the inhibition of the enzyme urease for the detection of heavy metals found in the mucus of stingrays from Laguna de La Paz, B.C.S. The self-assembly stages of the biosensor were characterized by Fourier Transform Infrared Spectroscopy (FTIR) in micro mode, with this technique was possible observe spectral variations associated to the presence of the functional groups corresponding to each of the stages of the biosensor self-assembly and the detection of heavy metal present in the regions between 1000 and 1200 cm-1 (C-O bonds), 1500 and 1700 cm-1 (amide I and II bonds) and 2800 and 3500 cm-1 (C-H and N-H bonds). Finally, we correlated the results with a principal component analysis, with this technique we were able to observe the clustering of the experimental data in function of each stage of the biosensor self-assembly and the concentration of the heavy metal present.
Palabras clave: stingray; Metals
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