Brassea-Pérez, E., V., Labrada-Martagón, C.J., Hernández-Camacho, R., Gaxiola-Robles, J.P., Vázquez-Medina & T., Zenteno-Savín (2023). DEHP exposure impairs human skeletal muscle cell proliferation in primary culture conditions: preliminary study. Cytotechnology. 75: 335–348. DOI: 10.1007/s10616-023-00580-4.
Elizabeth Brassea-Pérez 1, Vanessa Labrada-Martagón 2, Claudia Janetl Hernández-Camacho 3, Ramón Gaxiola-Robles 4, José Pablo Vázquez-Medina 5 y Tania Zenteno-Savín 6
The plasticizer di (2-ethylhexyl) phthalate
(DEHP) inhibits differentiation, impairs glucosemetabolism,
and decreases mitochondrial function in murine
muscle satellite cells; however, if these effects aretranslated
to human cells is unknown. The goal of this study
was to evaluate changes in morphology andproliferation
of primary human skeletal muscle cells exposed to
DEHP. Rectus abdominis muscle samples wereobtained
from healthy women undergoing programed cesarean
surgery. Skeletal muscle cells were isolated and grown
under standard primary culture conditions, generating
two independentsample groups of 25 subcultures
each. Cells from the first group were exposed to 1
mM DEHP for 13 days and monitored for changes in
cell morphology, satellite cell frequency and totalcell
abundance, while the second group remained untreated
(control). Differences between treated and untreated
groups were compared using generalized linear mixed
models (GLMM). Cell membrane and nuclear envelope
boundary alterations, loss of cell volume and presence
of stress bodies were observed in DEHP-treatedcultures.
DEHP-treated cultures also showed a significant
reduction in satellite cell frequency compared tocontrols.
Exposure to DEHP reduced human skeletal muscle
cell abundance. Statistical differences were found
between the GLMM slopes, suggesting that exposure to
DEHP reduced growth rate. These results suggest that
exposure to DEHP inhibits human skeletal muscle cell
proliferation, as evidenced by reduced cell abundance,
potentially compromising long-term culture viability.
Therefore, DEHP induces human skeletal muscle cell
deterioration potentially inducing an inhibitoryeffect of
myogenesis bydepleting satellite cells.
Palabras clave: Citotoxixidad; contaminantes emergentes; Ptalatos; cultivo primario
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