Herrera-Herrera, N.V., C.J., Band-Schmidt, I., Leyva-Valencia & L.M., Durán-Riveroll (2023). Allelopathic effect on competition between the epibenthic dinoflagellates Amphidinium thermaeum and Coolia malayensis in laboratory conditions. 20th International Conference on Harmful Algae. Hiroshima, Japón, noviembre 5 - 10, 2023, 23.
Nadia Valeria Herrera-Herrera 1, Christine Johanna Band-Schmidt 1, Ignacio Leyva-Valencia 2 y Lorena María Durán-Riveroll 3
The genera Amphidinium and Coolia are typical co existing species in marine benthic ecosystems. They produce a wide range of secondary metabolites within variable amounts of mucilage that may cover their substrate ; h owever, the control mechanism of colonization and competition is unclear W e propose that it m ight be thr ough allelopathic interactions. In the current study, the allelopathy between Amphidinium thermaeum and Coolia malayensis both isolated from Bahía de La Paz, Gulf of California w ere analyzed . Three experiments were performed using 300 mL allelopathy flasks: i) mixed cultures, ii) both species cultured without cell contact (separated by a membrane of 5 µm ), and iii) cultures with the addition of 50 and 100% of cell free filtrates. For all bioassa ys, cells of each species were harvested during the exponential growth phase. All experiments were inoculated with initial densities considering cell biovolume ratios of 1:1 and 1:2. The results showed the dominance of A. thermaeum in mixed cultures, as well as the effects on growth and morphological changes in C. malayensis (loss of motility, chlorosis, ecdysis and cell lysis). S imilar effects were observed in cultures exposed to cell filtrates without dilution . In cultures withou t cell contact, no allelopathic effects were observed in any of the species. These species appear to compete for space through allelopathy, mainly through contact, which is reflected in a decrease in cell density. Mucilage plays a direct and possibly activ e role in the transport of allelochemicals by increasing the surface of contact.
Palabras clave: allelopathy; benthic dinoflagellates; Amphidinium; Coolia
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