Sánchez, M., P., Gallardo, P., Domingues, C., Rosas, C., Pascual & B.P., Ceballos-Vázquez (2023). Changes in digestive enzymes and nutritional ontogeny reserves in newly hatched Pacific pygmy octopus, Paroctopus digueti. Aquaculture. 576: 739873. DOI: 10.1016/j.aquaculture.2023.739873.
Magalli Sánchez 1, Pedro Gallardo 2, Pedro Domingues 3, Carlos Rosas 2, Cristina Pascual 2 y Bertha Patricia Ceballos-Vázquez 4
In this study, changes in digestive physiology and nutritional reserve content during theearly stages of the Pacific pygmy octopus, Paroctopusdigueti,were examined. Body growth, biochemical composition(soluble protein and glycogen concentrations) in the digestive gland and armmuscle, hepatosomatic index, as well as lipases and proteases (acid andalkaline) activities were determined every two days up until 14 days after hatching. Newly hatched octopuses were obtainedin the laboratory, from the eggs of a female that spawned in the wild and werekept in individual chambers and fed fresh crab meat. A significant decrease insoluble protein was observed in both, the digestive gland (8 days afterhatching) and the arm muscle (2-4 days after hatching). Thereafter, solubleprotein gradually increases in digestive gland whereas, in the arm muscle, afterwarda significant increase at 6 days after hatching, a decreasing pattern until 14 daysafter hatching was noticed, possibly due to the start of exogenous feeding. In armmuscle, 2 days after hatching the glycogen showed a peak with subsequent markedfluctuations up to 14 days after hatching, indicative of the complete use ofinternal nutritional reserves and dependence on exogenous feeding at this time.Higher lipase activity was observed 2 days after hatching, with a significantdecrease occurring between 6 and 14 days after hatching. Acid protease activityincreased between 6 and 14 days after hatching, while alkaline proteaseactivity remained stable during the developmental period, with a peak occurring8 days after hatching. These results contribute to the further understanding ofthis critical period of transition from endogenous to exogenous feeding thattakes place between 6 and 8 days after hatching and may be useful in developingeffective feeding schemes employing natural or formulated foods for thisspecies.
Palabras clave: cephalopod; digestive gland; digestive enzymes; internal reserves; Octopus culture
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