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¹ Instituto Politécnico Nacional, Centro Interdisciplinario de Ciencias Marinas

The plasticizer bis (2-ethylhexyl) phthalate (DEHP) dysregulates reactive oxygen species (ROS) production and antioxidant defenses. Despite all the available information on the hazardous effects of DEHP in mammals, its potential impact in skeletal muscle remains unclear. The objective of this study was to assess changes in redox metabolism induced by DEHP in human skeletal muscle cells in primary culture. After obtaining informed consent, rectus abdominis muscle samples were collected from healthy women (n=5) undergoing programed cesarean surgery at term. Skeletal muscle cells were isolated and grown under standard cell culture conditions. Cells were exposed to 1 mM of DEHP (treated group) for 13 days; cells maintained without DEHP were considered as the control group. Superoxide radical (O₂^•?) production, superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR), glutathione peroxidase (GPx) and glutathione S-transferase (GST) activities, and thiobarbituric acid reactive substances (TBARS) concentration were quantified using spectrophotometric methods. Exposure to 13 days of DEHP (1 mM) increased O₂^•? production, SOD, GPx, GR and GST activities as well as TBARS levels (p<0.05). These results show DEHP induces oxidative stress in human skeletal muscle cells in primary culture. Overproduction of O₂^•? and increased lipid peroxidation could exacerbate degenerative processes in skeletal muscle, such as sarcopenia, leading to an increase of cell death.

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