Rosales Velázquez, M.O., I., Lopez Silva & J.L., Ortiz Galindo (2022). Effect of water temperature on embryonic development and hatching time of the barred sand bass Paralabrax nebulifer (Perciformes: Serranidae). World Aquaculture 2021. Mérida, Yucatán, México, mayo 24 - 27, 2022, 1.
Martín Oscar Rosales Velázquez 1, Irlanda Lopez Silva 2 y José Luis Ortiz Galindo 1
In recent decades, aquaculture has been one of the animal origin food production sectors with the highest growth rate worldwide. The barred sand bass Paralabrax nebulifer, is one of the most important sources of small-scale commercial fisheries which represents the largest economic stream for cooperatives in Baja California Sur. Being a species of great fishery importance, it can also be said that this species can also be of aquacultural importance. The temperature represents a directive factor for the survival and growth of many aquatic organisms, the intention is to evaluate and determine an adequate temperature at which a better survival of embryos during incubation of the barred sand bass can be observed. Eggs were obtained by voluntary spawning,the incubation of embryos were carried out at controlled water temperatures (23, 25,27, and 29 oC). In order to determine the optimal incubation temperature a system was constructed consisting of 4 incubators each at different temperatures. Four steel inserts were used to attain the water temperature of 23 °C, this was regulated through the control of ambient temperature with the help of an air-conditioning mini-Split in automatic mode, were used resistance immersion heaters to increase the temperature to 25, 27, and 29 °C. The incubators were maintained in constant aeration for a better dispersion of temperature thought out the water. The embryos were randomly selected and transferred in 24-well microtiter plates (one embryo per well). Each well was filled with3.0 ml UV-sterilized sea water and were taken to the incubation area, eachtemperature treatment was replicated threetimes. During the incubation, the photo period (13:11 L:O) was kept up with neon lights. To ensure accuratemeasurements of the time intervals, themicrotiter plates were examined every 6hours to determine the hatching status andhealth of the embryos and larvae, for whicha dissecting microscope was used. Toobtain the LD50 (average lethal dose) afterwhich 50% of the organisms died a binary logistic regression “probit” was appliedusing the Minitab program. According to survival probality programthe LD50 was 22.4 °C (Figure 1). Thisindicates that 50% of the embryos survived to this temperature. The incubation periodis particularly sensitive to extremetemperatures within the tested temperaturerange. From a probabilistic perspective, the binarylogistic model revels that at 22.4 degrees,an organism has a 50% of proportionsurvival.
Palabras clave: Captive breeding; embryo development, incubation temperature, osmoregulation, hatching, barred sand bass grouper
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