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Resumen del producto

Martínez-Mercado, M.A., S., Z. Herzka, S.P.A., Jiménez-Rosenberg, J.C., Compaire, C.E., Galindo-Sánchez & L., Barbero (2022). Increasing spawning ground knowledge for marine fishes through metabarcoding. 45th Annual Larval Fish Conference & 13th International Larval Biology Symposium. La Jolla, CA, Estados Unidos de América, agosto 29 - septiembre 1, 2022, 1.

Increasing spawning ground knowledge for marine fishes through metabarcoding

Miguel A. Martínez-Mercado 1, Sharon Z. Herzka 1, Sylvia Patricia Adelheid Jiménez-Rosenberg 2, Jesús C. Compaire 3, Clara E. Galindo-Sánchez 1 y Leticia Barbero 4

1 CICESE
2 Instituto Politécnico Nacional, Centro Interdisciplinario de Ciencias Marinas, CICIMAR-IPN
3 Universidad de Buenos Aires, Argentina
4 NOAA’s Atlantic Oceanographic and Meteorological Laboratory, USA
Characterization of fish egg assemblages contributes to the identification of spawning grounds. Thisinformation is useful for biodiversity assessments, ecological early life history studies, and fisheriesmanagement. However, species-level identification based on the egg morphological characters ischallenging or impossible. Molecular identification using genetic markers (barcoding) like cytochromeoxidase I (COI) has improved the taxonomic resolution achieved for individual eggs. We implement highthroughputsequencing of barcodes (metabarcoding) for fish egg assemblages collected throughout theGulf of Mexico during the GOMECC-3 cruise in the summer of 2017 with oblique bongo tows with 335um nets. Stations included shallow (ca. 20 m) shelf stations as well as the deep-water region (bottomdepth > 1000 m). A ~313 bp COI amplicon was used in 19 fish eggs-sorted samples containing 4,771 eggsin sum. Collectively, 108 species were identified, corresponding to 82 genera and 43 families. Weanalyze the diversity, species composition, community structure, occurrence, and the relationshipbetween species presence/absence and sea surface temperature and salinity. Overall, we show howmetabarcoding can be successfully applied to broad spatial scale studies of natural marine fish eggassemblages leading to expanding our knowledge on marine fish spawning.

Palabras clave: Fish egg; metabarcoding; Gulf of Mexico

Para obtener una copia del documento contacta la personal de la biblioteca a través del correo bibliocicimar{a}ipn.mx

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