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Benthic ecosystems are regulated by secondary metabolites, which in turn are regulated by space and substrate type. In this work, growth interactions of the epibenthic dinoflagellates Amphidinium and Coolia were evaluated. Isolates from Bahía de La Paz, Gulf of California were cultured in GSe medium at 24 ± 1 °C, at a salinity of 34, under a 12:12 h L:D cycle at 150 µmol photons m-2 s-1. Growth curves were performed in 300 mL flasks, 1000 and 240 µL plates. Growth interaction cultures were followed in 200 µL with the following cell biovolume proportions: 1:1, 1:2, and 2:1 for 12 days. Molecular identification was performed by amplifying the D1-D3 region of the LSU-rDNA, and identified as A. thermaeum and C. malayensis. The highest biomass of both species was observed in 1000 µL in comparison to 240 µL and 300 mL (5.58 x 104 ± 1.18 x 104 and 1.77 x 104 ± 1.34 x 104 cells mL-1; 9.72 x 103 ± 8.57 x 102 and 133 ± 72 cells mL-1; 1.7 x 104 ± 2.01 x 103 and 9.78 x 103 ± 2.52 x 103 cells mL-1, respectively). None of the species were morphologically affected by the interaction; growth rates were similar to the controls (0.4 - 0.5 div day-1). A. thermaeum predominated in all proportions. Cell densities decreased compared to the controls in both species, which was more evident in the 1:1 proportion (2.59 x 104 ± 1.06 x 104 and 163 ± 131 cells mL-1, respectively). These species do not seem to be involved in allelopathic mechanisms; however, they compete for space resulting in a decrease in cell density. Estimating the growth and effects of epibenthic dinoflagellates is challenging due to their adhesion capacity, swimming speed, mucilage production, and life cycle complexity.

Palabras clave: allelopathy; Epibenthic dinoflagellates; Growth

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