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Resumen del producto

Fernández Herrera, L., C.J., Band-Schmidt, T., Zenteno-Savín, I., Leyva-Valencia, C.J., Hernández-Guerrero, M., Muñoz-Ochoa, A.R., Díaz-Marrero & J.J., Fernandez-Castro (2021). Cell death and metabolic stress in Gymnodinium catenatum induced by allelopathy of Chattonella marina var. marina , Gymnodinium impudicum and Margalefidinium polykrikoides. 19th International Conference on Harmful Algae (ICHA 2021). La Paz, Baja California Sur, México, octubre 10 - 15, 2021, 98.

Cell death and metabolic stress in Gymnodinium catenatum induced by allelopathy of Chattonella marina var. marina , Gymnodinium impudicum and Margalefidinium polykrikoides

Leyberth Fernández Herrera, Christine Johanna Band-Schmidt, Tania Zenteno-Savín, Ignacio Leyva-Valencia, Claudia Judith Hernández-Guerrero, Mauricio Muñoz-Ochoa, Ana R. Díaz-Marrero y Jose J. Fernandez-Castro

Allelopathy between phytoplankton species can induce cell stress and when the caspase-type protease enzymes are activated, they can promote apoptosis or induce programmed cell death (PCD) signals in the cells. Chattonella marina var. marina, Margalefidinium polykrikoides and Gymnodinium impudicum have an allelopathic effect on Gymnodinium catenatum, the physiological responses of these interactions are unknown. We evaluated whether the allelopathic effect promotes cellular stress and activates PCD in G. catenatum. Cultures of G. catenatum with a cell density of 500 cells mL-1 were exposed to C. marina var. marina, M. polykrikoides and G. impudicum cell-free media, during 24, 48 and 72 h. The mortality, superoxide radical O2 ?- production, thiobarbituric acid reactive substances (TBARS) levels, superoxide dismutase (SOD) activity, protein content, caspase-3 activity, and preliminary identification of metabolic changes by nuclear magnetic resonance (NMR), were evaluated in G. catenatum. The cell-free media from three species caused a mortality from 59 to 69 % in G. catenatum at 72 h. The maximum production of (O2 ?-) occurred with the cell-free medium of C. marina var. marina, while the highest TBARS production, and the highest SOD activity were induced by the culture medium of G. impudicum. The cell-free media of M. polykrikoides caused an increase in the total protein content. All treatments increased caspase-3 activity; in addition, changes were observed in the metabolism of G. catenatum. We conclude that the allelopathic effect on G. catenatum is related to cellular stress and the activity of caspase-3 as a possible executor of PCD. 

Palabras clave: dinoflagellates; allelopathy; programmed cell death; ROS; caspasa

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