Resumen del producto

The Gulf of California is recognized as an area with a high primary productivity. Dinoflagellate species of the genus Alexandrium such as A. catenella and A. ostenfeldii, that produce hydrophilic and lipophilic toxins inhabit in this region. In April 2019, from a water sample collected in southern Bahía de La Paz individual cells were identified morphologically as Alexandrium pseudogonyaulax. To corroborate the identification, monoclonal cell cultures were grown with GSe medium, at 24 ± 1°C at a salinity of 34, under a 12:12 h L:D cycle at 150 µmol photons m¯²s¯¹. Molecular identification was performed amplifying rDNA regions: 28S, 18S and 5.8S, with rDNA universal primers. The length of the sequences was 682 bp, 1657 bp, and 493 bp, respectively. The algorithms Maxima Parsimonia Maximum Likelihood and Bayesian Inference were used to perform phylogenetic analyzes. The best substitution model selected for 28S and 5.8S was TrN + G, and GTR + G for 18S sequences. The strain from Bahía de La Paz was included within the A. pseudogonyaulax clade, with all the molecular markers. Alexandrium pseudogonyaulax produces Goniodomin A, a lipophilic toxin with hemolytic activity, this species is recognized as ichthyotoxic. In Mexico A. pseudogonyaulax has been reported in the Pacific coast and the Gulf of California, although there is no other molecular data or toxinology studies in this species. This strain will be used to determine its toxin profile, toxicity in animal and cellular models, as well as for allelopathic interactions with other microorganisms.

Palabras clave: dinoflagellate; Bahía de La Paz; sequences

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