Resumen del producto

In Bahía de La Paz, the presence of harmful species of the genus Prorocentrum have been registered, nevertheless few studies have focused on their ecophysiology and identification. The objective of this work was to identify at species level four strains of Prorocentrum and cultivate them in two culture media. This study will allow us to improve the monitoring of HAB to decide on which species focus attention. Morphological and molecular identification was carried out with light microscopy, scanning electron microscopy, and also by using partial sequences of the 28S region of rDNA LSU. Growth curves were followed in f/2 and modified GSe media with vermicomposting extract, maintaining a temperature of 24 ± 1 °C, salinity of 34, light intensity of 120 - 150 µmol photon m-2 sec-1 and a photoperiod of 12:12 h light:dark. Strains were identified as P. rhathymum / P. mexicanum, P. koreanum and P. cassubicum. The maximum biomass for P. rhathymum / P. mexicanum was 52,177 ± 1,415 cells mL-1, for P. rhathymum / P. mexicanum 52,830 ± 4,592 cells mL-1and for P. koreanum 101, 974 ± 9,138 cells mL-1in GSe medium; with exponential growth rates of 0.599 ± 0.001 div day-1, 0.360 ± 0.003 div day-1, and 0.714 ± 0.005 div day-1, respectively. For P. cassubicum the highest maximum biomass (74,312 ± 2,008 cells mL-1) and growth rate (0.464 ± 0.006 div day-1) were observed in f/2 medium. Toxin profile analyzes are required to complement the characterization of these species. The correct taxonomic identification of Prorocentrum species is essential information for harmful algae monitoring programs. 

Palabras clave: prorocentrum; Growth; Bahía de La Paz

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