Resumen del producto

In this study, we analysed the calibration of batch fecundity assessment obtained by the gravimetric, Weibel-Gomez, and physical dissector methods, when applied to the same group of mature Pacific sardine females (Sardinops sagax) a species with indeterminate fecundity, for which the production of oocytes per female can only be estimated each spawning event. In all of the applied methods, digital image processing techniques were used. To apply the Weibel-Gomez method, micrometric estimates of the shape and size of the oocytes were used to preclude the necessity for assumptions regarding any of these parameters. Much of the bias was related to the tissue shrinkage due to the histological technique, so we estimated a correction factor that was applied to all of the batch fecundity estimates. In addition, further bias was introduced in the physical dissector method because the density of intraovaric oocytes at final maturity influences the dissector based on its depth of acquisition, so a geometric system was developed to reduce this bias.

Palabras clave: Sardinops sagax; stereology; Fecundity

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