Resumen del producto

Ocean organisms live in competitive environments that demand the production of poisons and toxins. In somecases, these substances have been used in the pharmaceutical industry for human disease treatments. Most fishpoisons generally have potent cytolytic activity, probably through cardiovascular and neuromuscular effects. Inthe case of marine stingrays, the injuries made by their tail venom apparatus are caused by the mechanical penetrationof their sting and a subsequent venom release. This study focused on the evaluation of substances withcytotoxic activity in the epithelium that covers the venom apparatus from the marine stingray Hypanus dipterurus.To demonstrate the above, the hemolytic, proteolytic and cytotoxic capacities of H. dipterurus epitheliumsubstances were determined. Discs impregnated with epithelial extract were used on blood agar plates. The proteolyticactivity was analyzed using casein as substrate and for gelatin the liquefaction activity test. To determinethe cytotoxicity degree of the extracts, the proliferation and cell viability MTT bioassay was implemented on humancervical carcinoma cells (HeLa). The results showed that no hemolytic or proteolytic activity existed againstcasein associated with the epithelial extract, but gelatin hydrolysis and cytotoxic activity against the HeLa cellline were observed. This study concludes that the substances found in the epithelium covering the H. dipterurusstingray venom apparatus are a mixture of various proteins, among which, glycosylated anionic proteins representa potential source of molecules with cytotoxic and hydrolytic activity.

Palabras clave: Cytotoxicity; Venom apparatus; Epithelium; Hypanus dipterurus; stingray

Para obtener una copia del documento contacta la personal de la biblioteca a través del correo bibliocicimar{a}ipn.mx