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Rubio-Castro, G.G., A., Munguía-Vega, C., Quiñonez-Velázquez & F.J., García Rodríguez (2018). Isolation and characterization of 21 novel microsatellite loci in sailfish Istiophorus platypterus Shaw 1792 from a shotgun genomic library. Journal of Applied Ichthyology. 34(4): 974-976. DOI: 10.1111/jai.13686.

Isolation and characterization of 21 novel microsatellite loci in sailfish Istiophorus platypterus Shaw 1792 from a shotgun genomic library

Griselma Guadalupe Rubio-Castro, A. Munguía-Vega, Casimiro Quiñonez-Velázquez 1 y Francisco Javier García Rodríguez 1

1 Instituto Politécnico Nacional, Centro Interdisciplinario de Ciencias Marinas
In the Eastern Pacific Ocean, catch records show high abundance of Istiophorus platypterus from the Gulf of California to Ecuador (Kume & Joseph, 1969). Sailfish is an important resource for commercial and recreational fishing in Mexico, and accounts for about 40% of the total catch for billfish, over 50% of the catch for recreational fishing and can reach up to 90% in some ports (Arias-Olaiz, 2007), also represent a considerable economic income for the country. Nevertheless, few studies have been conducted about the structure and genetic diversity of sailfish, employing mitochondrial DNA markers, and microsatellite loci developed for blue marlin Makaira nigricans (Buonaccorsi & Graves, 2000; Lu, Alvarado-Bremer, McKenzie, & Chiang, 2015; McDowell & Graves, 2002; Rubio-Castro, Quiñonez-Velázquez, & García-Rodríguez, 2016). The amount and spatial scale of genetic structure provides useful information for the management and conservation species, but the development of species-specific molecular markers is essential to avoid potential issues with cross-amplification (Selkoe & Toonen, 2006). Microsatellites (simple sequence repeats, SSR) are one of the most widely used molecular markers for population genetics, fisheries, paternity testing, molecular breeding and conservation (Oliveira, Padua, Zucchi, Vencovsky, & Vieira, 2006). Although I. platypterus is one of the most important fishery resources, microsatellites for this species had not been described before. In this study, we isolated and characterized the first set of microsatellite markers for I. platypterus from a shotgun genomic library.

Palabras clave: Sailsfish; microsatellites; Eastern Pacific

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