Resumen del producto

Beyond providing insights into developmental processes, physiological responses, and evolutionary questions, genomic and other ‘omic approaches can provide essential data for stock assessment models for a given species. Recently, these tools were successfully used to provide both biological data and population structure resolution to help inform stock models for species such as the Atlantic Herring, Turbot, and Atlantic Cod. Similarly, we are using genomic sequencing to develop sex-specific markers for two important commercial species, California Yellowtail (Seriola dorsalis) and Albacore Tuna (Thunnus alalunga). These markers will be indispensable for identifying sex either in immature fish or in individuals sampled non-destructively (i.e., fin clips from a tagged fish). This information will be particularly important for Albacore, where sex data could be key to improving current stock assessment models. In the Yellowtail project, we conducted genome-wide resequencing to identify genomic regions linked to sex-determination, while simultaneously screening populations for signals of local adaptation. A total of 90 wild male and female Yellowtail were collected from three locations in the eastern Pacific: coastal San Diego in Southern California, Cedros Island offshore of central Baja California, and La Paz in the southern portion of the Gulf of California in Baja California Sur. These sampling areas represented different oceanographic environments (e.g., temperature, salinity) and span the core range of S. dorsalis in the eastern Pacific. Using this approach, a single sex-determining region within the Yellowtail genome has been identified, and a putative sex-marker has been developed and is undergoing validation. Populations of Yellowtail also exhibited signals of local adaptation in several genes. For the Albacore project, a draft genome is being assembled for male and female individuals. Additionally, genome-wide resequencing was conducted on 20 individuals (10 of each sex) to help identify the sex-determining region in this species. This presentation will summarize the results of both projects, to date.

Palabras clave: Stock assessment; genomic; yellowtail

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