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Resumen del producto
Max Aguilar, A., A., Munguía Vega, N., Diaz Viloria & R., Pérez Enríquez
(2014).
Development and characterization of new microsatellte lociin green abalone (Hallotls fulgens).
Mollusca 2014, The meeting of the Americas.
México, DF, Mexico, México, junio 22 - 27, 2014,
138-139.
Development and characterization of new microsatellte lociin green abalone (Hallotls fulgens)
Adriana Max Aguilar, Adrián Munguía Vega, Noe Diaz Viloria y Ricardo Pérez Enríquez
Microsatellite markers are DNA sequences with 2-5 nucleotide repeats useful for population genetics studies and parentage assessment, of which tetranucleotide repeats have shown adequate levels of polymorphism and clear genotyping patterns. Microsatellites were development for the green abaloneHaliotis fulgens via 454 high-throughtput sequencing. Primers for PCR amplification weredesigned for a set of 80 tetranucleotide loci. Twenty-four microsatellite loci potentially useful in genetic analysis were obtained, from which 12 polymorphic loci have been characterized in a wild population sample (N=59). From these, 11 are considered useful in paternity analysis and population studies due to their high polymorphism (number of alleles: 6-16; HE: 0.73-0.91; PIC: 0.69-0.90) and beca use they were in Hardy Weinberg equilibrium (EHW). To increase the number of available loci, the other 12 microsatellites are being tested for polymorphism and HWE.
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