Resumen del producto

Because their high diversity and abundance in the oceans, the copepods have the potential to be intermediate hosts or paratenic of helminthes. Among the helminths stand out Anisakidae family, however, the early stages of the life history of these nematodes are poorly documented. In this study we experimentally induce infection in Calanoid copepods with Contracaecum multipapillatum (sensu lato) embryonated eggs. Five birds (Pelecanus occidentalis) were dissected and 660 nematodes were extracted. The nematode eggs were obtained by dissecting the uterus of adult females. Copepods Centropages furcatus, Acartia clausi and A. lilljeborgii species were used to experimental design and were obtained with a conical plankton net, in La Ensenada and Bahia de La Paz. From UPIMA facility at CICIMAR, individuals in culture of Pseudodiaptomus euryhalinus were obtained. The experimental design was a single factor with three treatments with two replicates each. The temperature and feed concentrations were constant. Daily hatching rate and infection was determined and recorded on video and photography. The first larva is developed between 6 and 8 days at 25 ° C, molt inside the egg and grows to the second stage between the eighth and tenth day period in which was hatch spontaneously, presenting a free-living stage. The wild copepods were infected with L2 larva, and significant differences between the concentrations were found (Kruskal-Wallis p <0.05). In contrast, no Pseudodiaptomus euryhalinus infestations were recorded. These preliminary results suggest that the wild species could act as parathenic or transport hosts in the life cycle of C. multipapillatum.

Palabras clave: Baja California Sur; copepods; Nemathod; infestation

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