Resumen del producto

Copepods have a large number of sibling species which show subtle morphological differences, and depending of the Order and geographical region, differences could be more or less visible. The accurate species identification is the basis of any ecological study, but sometimes this basic step is not met due to several factors as unrecognized larval instars, or juveniles, similar morphology or lack of identification keys, drawings or any other valid source. Currently the Taxonomy studies, not only of copepods, in order to help to identify species uses the molecular approach when is not easy to identify using morphological characters; several genes have been used, among which is that decodes for cytochrome c oxidase 1 (CO1). Molecular methods have been used to obtain sequences for several species of copepods worldwide, and in Mexico the association of morphology with genetics using CO1 is increasing. In order to relate the Linnaean taxonomy with CO1 sequence of Pontellidae family in the Gulf of California, results are presented. Ten species have been identified morphologically, of which Calanopia elliptica, Calanopia minor, Pontellopsis armata and Pontellopsis brevis are new records for the study area. Gene sequences of Calanopia elliptica, Pontellopsis armata, Labidocera acuta, Labidocera acutifrons, Pontellina plumata and Labidocera johnsoni were obtained. The analysis shows that the two-parameter Kimura model, or K2P, at species level had maximum divergence of 1.39%, low of 0% and averaged 0.14%, while at genus level the maximum divergence was 24,023 %, with a low of 21.28% and 22.44% average. Bootstrap values on nodes that group the species are high (95.7% to 100%) which indicates good consistency. The greatest differences were observed in Pontellina plumata and minor in Labidocera johnsoni and Calanopia elliptica, the latter also had a difference of 0% so that absent intraspecific variation, confirming the identity of individuals of these species. So far, the usefulness of this tool can be linked successfully molecular morphology of copepods.

Palabras clave: pontellidae; Paralarval assemblages; copepods; Barcoding

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