Resumen del producto

In spite of the success in reducing ozone-depleting emissions, environmental UV is not expected to decrease for several decades. Thus, it is pressing to implement tools useful for investigating the capacity of wildlife to respond to excessive or constant UV exposure, particularly of species that lack anatomo-physiological protection against UV such as cetaceans. One approach, ideal in terms of its relative ease and minimal impact on large free ranging species that cannot be manually restrained, is to examine epidermal expression of key genes involved in genotoxic stress response pathways. However, accurate quantitation of mRNA transcripts requires previous standardization, not limited to including accurate selection of internal control genes, but also involving a careful choice of target genes. The latter is particularly important when working with environmental stressors such as UV, which can activate many different genes.

Palabras clave: Whales; Gene expression; qPCR normalization; Internal control genes; HSP70; P53; KIN; Solar ultraviolet radiation; Skin biopsy

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