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The application of natural antimicrobial compounds in prevention of marine biofouling has tremendous interest in the industry. Fouling organisms cause severe damage to the marine structures raised for commercial purposes. The key area in marine anti-foulant research, is finding compounds with activity to prevent formation of biofilms, the succession of flora they attract and also prevent bio-corrosion of ship hulls and submerged objects as a result of acid produced by anaerobic bacteria in marine biofilms. In the present study we report the potential of marine sponge bacteria associated to produce compounds with potential application as natural anti-foulants. A collection of 63 bacteria were isolated previously from Aplysina gerardogreeni sponge. The bacterial biomasses were extracted with hexane and ethyl acetate (60:40). Extracts were assayed for their antifouling activity against 16 marine and four terrestrial bacteria and five microalgae. The bioassay showed that 57 strains was active against any of the strains tested. The most sensitive strain was Bacillus subtilis, being inhibited by 69% of the extracts most with low MIC (0.01 ?mg ml-1). On the other hand, 37 extracts showed activity against any of the five strains of microalgae tested. The most sensitive was Cylindrotheca closterium, 29 extracts were able to inhibit their growth at low concentrations. Sequencing of partial 16 S ribosomal DNA analysis showed that the bacterial isolates from sponge A. gerardogreeni with more activity were related to Bacillus species. The results revealed that bacteria marine sponge associated are a potential source of novel antifouling agents.

Palabras clave: Bacteria; antifouling; extracts; sponge

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