Resumen del producto

The process to obtain fucoidan was analyzed for four species of phaeophyta: Sargassum sinicola, Macrocystis pyrifera, Lessonia trabeculata and Lessonia nigrescens. Algae were extracted three times with water at three temperatures (25, 55, and 85 ºC), with constant agitation. After each extraction, the fucoidan was precipitated with ethanol, recovered by centrifugation and dried at 55°C. Fucoidan yield (%) was computed for each fraction. The residual material was used to extract alginate in order to evaluate the effect on the yield, viscosity and gel strength for each experimental temperature. Fucoidan was tested as anticoagulant using the protombin time (PT) and activated partial tromboplastin time (APTT) assays. Phenol-H₂SO4 reaction was used to determine the total sugars content. Infrared spectra were obtained to evaluate the sulfate content. At all temperatures, the higher fucoidan percentage (60-80%) was recovered from the first fraction. The higher yield was obtained at 85°C, however 55°C is recommended because alginate properties were not affected. Fucoidan content by species was as follow: S. sinicola (11.86%), M. pyrifera (8.26%), L. trabeculata (4.38%), and L. nigrescens (3.20%). The PT (in seconds) for the control was 27.8. PT when using the fucoidan at the maximum concentration tested (333 ?g mL- 1) was: L. trabeculata 20.4, L. nigrescens 25.8, S. sinicola 31.2, and M. pyrifera 105.5. Same trend was obtained for the APTT. Control time was 27.8 s, and when using the fucoidan, time increased significantly, ranging from 147 to > 300 s. The phenol-H₂SO4 reaction detected sugars as the main component.

Palabras clave: Algae; APTT; prothronbin; Cuagulation time

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